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mcherry cdna  (TaKaRa)


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    Structured Review

    TaKaRa mcherry cdna
    Mcherry Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+c1/pmCherry-C1+Vector/pm41565995-276-20-26
    Average 96 stars, based on 3286 article reviews
    mcherry cdna - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles
    Article Snippet: .. The plasmid coding for mCherry fused to β-actin, driven by the Ubiquitin-C promotor (mCherry-actin-Ub) was generated in two steps: 1. mCherry-C1-Ub was generated from pUB-GFP (Addgene plasmid #11155) by inserting mCherry amplified from mCherry-C1 (Clontech) using 5’-CGGCATTAATGATCTGGCCTCCGCGCCGGGT-3′ and 5′-GGCCGCTAGCCGACCTGCAGCCCAAGCTTCGTC-3′, after digestion of both fragments with AseI/NheI, 2. mCherry-actin-Ub was generated from mCherry-C1-Ub by inserting β-actin amplified from mRFP1-actin using 5′-ATATGAATTCCGCCCCGCGAGCACAGA-3′ and 5′-ATATGGATCCTCAGTGTACAGGTAAGCCCTGGC-3′, after digestion of both fragments with EcoRI/BamHI. ..

    Article Title: Use of miR-18b for prevention, treatment, or diagnosis of muscle disease and neuromuscular disease
    Article Snippet: .. The amplified Mctp1 PCR product was cloned into mCherry C1 (Clontech) having a Hind III (NEW ENGLAND BioLabs) restriction enzyme site to prepare a Mctp1 plasmid construct. .. The amplified Rarb PCR product was cloned into eGFP N1 (Clontech) having Nhe I and Age I (NEW ENGLAND BioLabs) restriction enzyme sites to prepare a Rarb plasmid construct.

    Article Title: Programmable RNA base editing with photoactivatable CRISPR-Cas13
    Article Snippet: .. The constructs were generated using Gibson assembly, and DNA fragments corresponding to N351-pMag, nMagHigh1-C352, nMagHigh1-C350, EGFP-N1 (Takara), and mCherry-C1 (Takara) were inserted into the BamHI and NotI sites of the EGFP-N1 plasmid (Takara). .. The crRNAs targeting the Fluc reporter, the GFP indicator, NRAS, NFKB1, PPARG, KRAS , PPIB , STAT3 , and the A-to-I or C-to-U RNA editing sites were generated by annealed oligo cloning using the BbsІ site of the pC0043-PspCas13b crRNA backbone (Addgene plasmid 103854).

    Ubiquitin Proteomics:

    Article Title: Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles
    Article Snippet: .. The plasmid coding for mCherry fused to β-actin, driven by the Ubiquitin-C promotor (mCherry-actin-Ub) was generated in two steps: 1. mCherry-C1-Ub was generated from pUB-GFP (Addgene plasmid #11155) by inserting mCherry amplified from mCherry-C1 (Clontech) using 5’-CGGCATTAATGATCTGGCCTCCGCGCCGGGT-3′ and 5′-GGCCGCTAGCCGACCTGCAGCCCAAGCTTCGTC-3′, after digestion of both fragments with AseI/NheI, 2. mCherry-actin-Ub was generated from mCherry-C1-Ub by inserting β-actin amplified from mRFP1-actin using 5′-ATATGAATTCCGCCCCGCGAGCACAGA-3′ and 5′-ATATGGATCCTCAGTGTACAGGTAAGCCCTGGC-3′, after digestion of both fragments with EcoRI/BamHI. ..

    Generated:

    Article Title: Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles
    Article Snippet: .. The plasmid coding for mCherry fused to β-actin, driven by the Ubiquitin-C promotor (mCherry-actin-Ub) was generated in two steps: 1. mCherry-C1-Ub was generated from pUB-GFP (Addgene plasmid #11155) by inserting mCherry amplified from mCherry-C1 (Clontech) using 5’-CGGCATTAATGATCTGGCCTCCGCGCCGGGT-3′ and 5′-GGCCGCTAGCCGACCTGCAGCCCAAGCTTCGTC-3′, after digestion of both fragments with AseI/NheI, 2. mCherry-actin-Ub was generated from mCherry-C1-Ub by inserting β-actin amplified from mRFP1-actin using 5′-ATATGAATTCCGCCCCGCGAGCACAGA-3′ and 5′-ATATGGATCCTCAGTGTACAGGTAAGCCCTGGC-3′, after digestion of both fragments with EcoRI/BamHI. ..

    Article Title: Programmable RNA base editing with photoactivatable CRISPR-Cas13
    Article Snippet: .. The constructs were generated using Gibson assembly, and DNA fragments corresponding to N351-pMag, nMagHigh1-C352, nMagHigh1-C350, EGFP-N1 (Takara), and mCherry-C1 (Takara) were inserted into the BamHI and NotI sites of the EGFP-N1 plasmid (Takara). .. The crRNAs targeting the Fluc reporter, the GFP indicator, NRAS, NFKB1, PPARG, KRAS , PPIB , STAT3 , and the A-to-I or C-to-U RNA editing sites were generated by annealed oligo cloning using the BbsІ site of the pC0043-PspCas13b crRNA backbone (Addgene plasmid 103854).

    Amplification:

    Article Title: Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles
    Article Snippet: .. The plasmid coding for mCherry fused to β-actin, driven by the Ubiquitin-C promotor (mCherry-actin-Ub) was generated in two steps: 1. mCherry-C1-Ub was generated from pUB-GFP (Addgene plasmid #11155) by inserting mCherry amplified from mCherry-C1 (Clontech) using 5’-CGGCATTAATGATCTGGCCTCCGCGCCGGGT-3′ and 5′-GGCCGCTAGCCGACCTGCAGCCCAAGCTTCGTC-3′, after digestion of both fragments with AseI/NheI, 2. mCherry-actin-Ub was generated from mCherry-C1-Ub by inserting β-actin amplified from mRFP1-actin using 5′-ATATGAATTCCGCCCCGCGAGCACAGA-3′ and 5′-ATATGGATCCTCAGTGTACAGGTAAGCCCTGGC-3′, after digestion of both fragments with EcoRI/BamHI. ..

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
    Article Snippet: .. Construction of Plasmids and Transfection: DNA fragments encoding full-length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV-Myc-N (635 689, Clontech), pCMV-Flag-N (635 689, Clontech), pEGFP-C3 (6082-1, Clontech), mCherry-C1 (632 524, Clontech), pET-28a-C (69864-3, Novagen), and pGEX-4T-1 (27-4580-01, GE Healthcare Biosciences). ..

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs
    Article Snippet: .. DNA fragments encoding full‐length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV‐Myc‐N (635 689, Clontech), pCMV‐Flag‐N (635 689, Clontech), pEGFP‐C3 (6082‐1, Clontech), mCherry‐C1 (632 524, Clontech), pET‐28a‐C (69864‐3, Novagen), and pGEX‐4T‐1 (27‐4580‐01, GE Healthcare Biosciences). ..

    Article Title: Use of miR-18b for prevention, treatment, or diagnosis of muscle disease and neuromuscular disease
    Article Snippet: .. The amplified Mctp1 PCR product was cloned into mCherry C1 (Clontech) having a Hind III (NEW ENGLAND BioLabs) restriction enzyme site to prepare a Mctp1 plasmid construct. .. The amplified Rarb PCR product was cloned into eGFP N1 (Clontech) having Nhe I and Age I (NEW ENGLAND BioLabs) restriction enzyme sites to prepare a Rarb plasmid construct.

    Transfection:

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
    Article Snippet: .. Construction of Plasmids and Transfection: DNA fragments encoding full-length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV-Myc-N (635 689, Clontech), pCMV-Flag-N (635 689, Clontech), pEGFP-C3 (6082-1, Clontech), mCherry-C1 (632 524, Clontech), pET-28a-C (69864-3, Novagen), and pGEX-4T-1 (27-4580-01, GE Healthcare Biosciences). ..

    Expressing:

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
    Article Snippet: .. Construction of Plasmids and Transfection: DNA fragments encoding full-length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV-Myc-N (635 689, Clontech), pCMV-Flag-N (635 689, Clontech), pEGFP-C3 (6082-1, Clontech), mCherry-C1 (632 524, Clontech), pET-28a-C (69864-3, Novagen), and pGEX-4T-1 (27-4580-01, GE Healthcare Biosciences). ..

    Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs
    Article Snippet: .. DNA fragments encoding full‐length gene were amplified from HEK293T cells and individually subcloned into the following expression vectors: pCMV‐Myc‐N (635 689, Clontech), pCMV‐Flag‐N (635 689, Clontech), pEGFP‐C3 (6082‐1, Clontech), mCherry‐C1 (632 524, Clontech), pET‐28a‐C (69864‐3, Novagen), and pGEX‐4T‐1 (27‐4580‐01, GE Healthcare Biosciences). ..

    Article Title: KCNH6 Potassium Channel Regulates Assembling of Mitochondrial Complex I and Promotes Insulin Secretion in Islet Beta Cells
    Article Snippet: Mitochondrial glucose metabolism is critical for glucosestimulated insulin secretion and glucose homeostasis in pancreatic β cells.. We previously showed that KCNH6, a voltagedependent potassium (Kv) channel, participated regulation of insulin secretion in pancreatic β cells, however, its role in mitochondrial metabolism remains unclear.. Since we recently found that KCNH6 distributed in mitochondria, in this study, we investigated the role of KCNH6 in regulating mitochondrial function in pancreatic β cells by using a β cellspecific knockout (KCNH6βKO) mouse model. Proteomics analysis of islets indicated that multiple proteins involved in mitochondrial metabolism were dysregulated in islets of KCNH6βKO mice.

    Polymerase Chain Reaction:

    Article Title: The conformation of FOXM1 homodimers in vivo is crucial for regulating transcriptional activities
    Article Snippet: The cDNA fragments of FOXM1 NRD or TAD were PCR amplified from pCMV-FOXM1b (#SC128214, OriGene) using the respective primer pairs ( ) and Phusion High-Fidelity PCR Kit (#F553, Thermo Scientific TM ). .. The resulting PCR products were digested with EcoRI-HF (#R3101, NEW ENGLAND BioLabs) and BamHI (#R3136, NEW ENGLAND BioLabs), and then ligated into mCherry-C1 (#632524, Clontech), EGFP-C2 (#6083–1, Clontech), ECFP-C1 (#6076–1, Clontech), EYFP-C1 (#6006–1, Clontech), pGAD424 (#U07647, Clontech) or pBTM116 ( ) vectors using T4 DNA ligase (#M202, NEW ENGLAND BioLabs). .. The cyan fluorescent protein (CFP) or yellow fluorescent protein (YFP) tagged FOXM1 in transient expression plasmids was subcloned with NdeI (#R0111, NEW ENGLAND BioLabs), MfeI (#R3589, NEW ENGLAND BioLabs) and EcoRI to lentiviral plasmids pLAS2w.Ppuro or pLAS2w.Pneo (National RNAi Core Facility, Taiwan).

    Article Title: Use of miR-18b for prevention, treatment, or diagnosis of muscle disease and neuromuscular disease
    Article Snippet: .. The amplified Mctp1 PCR product was cloned into mCherry C1 (Clontech) having a Hind III (NEW ENGLAND BioLabs) restriction enzyme site to prepare a Mctp1 plasmid construct. .. The amplified Rarb PCR product was cloned into eGFP N1 (Clontech) having Nhe I and Age I (NEW ENGLAND BioLabs) restriction enzyme sites to prepare a Rarb plasmid construct.

    Clone Assay:

    Article Title: Use of miR-18b for prevention, treatment, or diagnosis of muscle disease and neuromuscular disease
    Article Snippet: .. The amplified Mctp1 PCR product was cloned into mCherry C1 (Clontech) having a Hind III (NEW ENGLAND BioLabs) restriction enzyme site to prepare a Mctp1 plasmid construct. .. The amplified Rarb PCR product was cloned into eGFP N1 (Clontech) having Nhe I and Age I (NEW ENGLAND BioLabs) restriction enzyme sites to prepare a Rarb plasmid construct.

    Construct:

    Article Title: Use of miR-18b for prevention, treatment, or diagnosis of muscle disease and neuromuscular disease
    Article Snippet: .. The amplified Mctp1 PCR product was cloned into mCherry C1 (Clontech) having a Hind III (NEW ENGLAND BioLabs) restriction enzyme site to prepare a Mctp1 plasmid construct. .. The amplified Rarb PCR product was cloned into eGFP N1 (Clontech) having Nhe I and Age I (NEW ENGLAND BioLabs) restriction enzyme sites to prepare a Rarb plasmid construct.

    Article Title: Programmable RNA base editing with photoactivatable CRISPR-Cas13
    Article Snippet: .. The constructs were generated using Gibson assembly, and DNA fragments corresponding to N351-pMag, nMagHigh1-C352, nMagHigh1-C350, EGFP-N1 (Takara), and mCherry-C1 (Takara) were inserted into the BamHI and NotI sites of the EGFP-N1 plasmid (Takara). .. The crRNAs targeting the Fluc reporter, the GFP indicator, NRAS, NFKB1, PPARG, KRAS , PPIB , STAT3 , and the A-to-I or C-to-U RNA editing sites were generated by annealed oligo cloning using the BbsІ site of the pC0043-PspCas13b crRNA backbone (Addgene plasmid 103854).



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    Image Search Results


    PARP inhibitors increase MMEJ at ISceI-mediated deDSBs. ( A, B ) Schematic ( A ) and experimental timeline ( B ) of MMEJ reporter used in panels (C)–(F), Figs and , , , and E, and , and and . Cutting the reporter with ISceI and repair by microhomology annealing to an in-frame, functional mCherry gene. Flow cytometry was used to identify the mCherry + cells (MMEJ+) within the BFP+ (ISceI+) population. ( C–E ) MMEJ quantification using reporter and timeline from panel (A) and (B) in HT1080 cells. Values are normalized to DMSO. Drugs used were olaparib (5 µM or as indicated), DNA-PKcsi (NU7441, 1 µM), Polθi (ART558 10 µM), niraparib (2.5 µM), rucaparib (2.5 µM), and talazoparib (2.5 µM). ( F ) MMEJ quantification in HT1080 parental cells compared to isogenic POLQ -KO cells following olaparib treatment. Values are normalized to wild-type DMSO. Statistical analyses for panels (C)–(F): Data represent three independent experiments, each the average of three technical replicates. Data are mean ± SEM. Statistical test, one way ANOVA with multiple comparison correction. ns: nonsignificant, * P <.05, ** P <.01, *** P <.001, **** P <.0001.

    Journal: Nucleic Acids Research

    Article Title: PARP1 and PARP2 are dispensable for DNA repair by microhomology-mediated end-joining at double-ended DSBs

    doi: 10.1093/nar/gkaf1437

    Figure Lengend Snippet: PARP inhibitors increase MMEJ at ISceI-mediated deDSBs. ( A, B ) Schematic ( A ) and experimental timeline ( B ) of MMEJ reporter used in panels (C)–(F), Figs and , , , and E, and , and and . Cutting the reporter with ISceI and repair by microhomology annealing to an in-frame, functional mCherry gene. Flow cytometry was used to identify the mCherry + cells (MMEJ+) within the BFP+ (ISceI+) population. ( C–E ) MMEJ quantification using reporter and timeline from panel (A) and (B) in HT1080 cells. Values are normalized to DMSO. Drugs used were olaparib (5 µM or as indicated), DNA-PKcsi (NU7441, 1 µM), Polθi (ART558 10 µM), niraparib (2.5 µM), rucaparib (2.5 µM), and talazoparib (2.5 µM). ( F ) MMEJ quantification in HT1080 parental cells compared to isogenic POLQ -KO cells following olaparib treatment. Values are normalized to wild-type DMSO. Statistical analyses for panels (C)–(F): Data represent three independent experiments, each the average of three technical replicates. Data are mean ± SEM. Statistical test, one way ANOVA with multiple comparison correction. ns: nonsignificant, * P <.05, ** P <.01, *** P <.001, **** P <.0001.

    Article Snippet: The PARP2 plasmid used to complement PARP2 -KO clones was a generous gift from the Karolin Luger lab and was derived by cloning the human PARP2 coding region into plasmid mCherry C1 (obtained from Dyche Mullins [ ], Addgene #58476).

    Techniques: Functional Assay, Flow Cytometry, Comparison

    PARPi-dependent MMEJ increase does not occur at Cas9-induced DSBs. ( A, B ) Schematic ( A ) and experimental timeline ( B ) of MMEJ reporter used in panels (C)–(E), Figs and . Repair of the Cas9-induced cut with microhomology annealing leads to an in-frame, functional mCherry gene. Flow cytometry was used to identify the mCherry + cells (MMEJ+) within the GFP+ (Cas9+) population. ( C–E ) MMEJ quantification using the reporter and timeline from panels (A) and (B) in HT1080 cells. Values are normalized to DMSO. Drugs used were olaparib (5 µM or indicated dose), DNA-PKcsi (NU7441, 1 µM), Polθi (ART558, 10 µM). ( F ) HR quantification using the DR-GFP reporter in HT1080 cells after an ISceI- or Cas9-induced DSB, ± olaparib treatment (5 µM). Statistical analyses ( C–F ): Data represent three (D–F) or four ( C ) independent experiments, each the average of three technical replicates. Data are mean ± SEM. Statistical test, one way ANOVA with multiple comparison correction. ns: nonsignificant, * P <.05, ** P <.01, *** P <.001, **** P <.0001.

    Journal: Nucleic Acids Research

    Article Title: PARP1 and PARP2 are dispensable for DNA repair by microhomology-mediated end-joining at double-ended DSBs

    doi: 10.1093/nar/gkaf1437

    Figure Lengend Snippet: PARPi-dependent MMEJ increase does not occur at Cas9-induced DSBs. ( A, B ) Schematic ( A ) and experimental timeline ( B ) of MMEJ reporter used in panels (C)–(E), Figs and . Repair of the Cas9-induced cut with microhomology annealing leads to an in-frame, functional mCherry gene. Flow cytometry was used to identify the mCherry + cells (MMEJ+) within the GFP+ (Cas9+) population. ( C–E ) MMEJ quantification using the reporter and timeline from panels (A) and (B) in HT1080 cells. Values are normalized to DMSO. Drugs used were olaparib (5 µM or indicated dose), DNA-PKcsi (NU7441, 1 µM), Polθi (ART558, 10 µM). ( F ) HR quantification using the DR-GFP reporter in HT1080 cells after an ISceI- or Cas9-induced DSB, ± olaparib treatment (5 µM). Statistical analyses ( C–F ): Data represent three (D–F) or four ( C ) independent experiments, each the average of three technical replicates. Data are mean ± SEM. Statistical test, one way ANOVA with multiple comparison correction. ns: nonsignificant, * P <.05, ** P <.01, *** P <.001, **** P <.0001.

    Article Snippet: The PARP2 plasmid used to complement PARP2 -KO clones was a generous gift from the Karolin Luger lab and was derived by cloning the human PARP2 coding region into plasmid mCherry C1 (obtained from Dyche Mullins [ ], Addgene #58476).

    Techniques: Functional Assay, Flow Cytometry, Comparison

    (A) Cells were transfected with the pmCherry-C1 actin-3×NLS P2A mCherry construct. After transfection, the cells were fixed with 3.7% paraformaldehyde and subjected to immunofluorescence staining for FNBP4. FNBP4 was detected using an anti-FNBP4 antibody, and nuclei were counterstained with DAPI. The representative image shows a single optical section from the middle of the Z-stack. Scale bar: 20 µm. (B) Quantification of FNBP4 and actin colocalization using Manders’ Colocalization Coefficient. The scatter dot plot shows the distribution of data points along with standard deviations.

    Journal: bioRxiv

    Article Title: Dual Regulatory Role of Nuclear FNBP4 in Actin Binding and Formin FMN1 Inhibition

    doi: 10.64898/2026.01.05.697755

    Figure Lengend Snippet: (A) Cells were transfected with the pmCherry-C1 actin-3×NLS P2A mCherry construct. After transfection, the cells were fixed with 3.7% paraformaldehyde and subjected to immunofluorescence staining for FNBP4. FNBP4 was detected using an anti-FNBP4 antibody, and nuclei were counterstained with DAPI. The representative image shows a single optical section from the middle of the Z-stack. Scale bar: 20 µm. (B) Quantification of FNBP4 and actin colocalization using Manders’ Colocalization Coefficient. The scatter dot plot shows the distribution of data points along with standard deviations.

    Article Snippet: For transfection studies, the pmCherry-C1 actin-3×NLS P2A mCherry plasmid was obtained from Addgene (RRID:Addgene_58475; deposited by Dyche Mullins).

    Techniques: Transfection, Construct, Immunofluorescence, Staining

    HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization with Omp25-mCherry ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.

    Journal: The Journal of Biological Chemistry

    Article Title: Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism

    doi: 10.1016/j.jbc.2025.111009

    Figure Lengend Snippet: HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization with Omp25-mCherry ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.

    Article Snippet: Cells were transfected with HK1-GFP (Addgene, 21917) or HK2-GFP (Addgene, 21920) along with Omp25-mCherry (Addgene, 157758) using Lipofectamine Stem transfection reagent (Thermo Fisher Scientific, STEM00008) according to the manufacturer's protocol.

    Techniques: Knock-Out